Speaker：Inv. Tang Chun

Time: Tuesday, Dec. 25, 2019, 10:00 -

Location: Room 3119，Building 403，Dushu Lake Campus of Soochow University

Abstract：

  Proteins are inherently dynamic. The dynamics allow the protein to fulfill their specific functions. NMR is exquisitely sensitive to protein dynamics in solution. In particular, paramagnetic NMR used to visualize rare protein conformation(s) that are populated at less than 5%. However, due to ensemble averaging of NMR signals over many copies of the protein molecule, it can be difficult to deconvolute the constituting conformational states that give rise to the observables. Single molecule techniques, on the other hand, can be used to visualize the fluctuation of a single protein molecule over time. In my presentation, I will show how the characterization of protein ensemble structures can be more readily accomplished with the incorporation of single molecule FRET data. The relative populations of the interconverting conformational states can be obtained from smFRET, and detailed structural information can be characterized using NMR, in particular paramagnetic NMR. In addition, we have used cross-linking coupled with mass spectrometry (CXMS) and small angle X-ray scattering (SAXS) in the lab for additional restraints of refine an ensemble of protein structures. Using both bulk and single-molecule measurements, we have visualized dynamic structures of poly ubiquitin and other multi-domain/subunit proteins. Together, the integration of other biophysical techniques augments NMR depiction of protein structure and dynamics.